Other

Part:BBa_J99001:Design

Designed by: David Bikard   Group: Mazel Lab   (2009-12-17)

attCaadA7 integron recombination site


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

PCR amplified with oligos 5'-GCAGAATTCGCGGCCGCTTCTAGAGGGTTATAACAATTCATTCAAGCCGACGCCGCTTCGCGGCGCGG-3' and 5'-AGCCTGCAGCGGCCGCTACTAGTAGGTTATAACGCTTGAATTAAGCCGCGCCGCGAAGCGGCGTCG-3'


Source

References

Mazel, D. et al. Antibiotic Resistance in the ECOR Collection: Integrons and Identification of a Novel aad Gene. Antimicrob. Agents Chemother. 44, 1568-1574(2000).

Biskri, L. et al. Comparative study of class 1 integron and Vibrio cholerae superintegron integrase activities. J Bacteriol 187, 1740-1750(2005).