Other
Part:BBa_J99001:Design
Designed by: David Bikard Group: Mazel Lab (2009-12-17)
attCaadA7 integron recombination site
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
PCR amplified with oligos 5'-GCAGAATTCGCGGCCGCTTCTAGAGGGTTATAACAATTCATTCAAGCCGACGCCGCTTCGCGGCGCGG-3' and 5'-AGCCTGCAGCGGCCGCTACTAGTAGGTTATAACGCTTGAATTAAGCCGCGCCGCGAAGCGGCGTCG-3'
Source
References
Mazel, D. et al. Antibiotic Resistance in the ECOR Collection: Integrons and Identification of a Novel aad Gene. Antimicrob. Agents Chemother. 44, 1568-1574(2000).
Biskri, L. et al. Comparative study of class 1 integron and Vibrio cholerae superintegron integrase activities. J Bacteriol 187, 1740-1750(2005).